Angelo Vertti, 18 de setembro de 2022

20. For ve-hicle control, 10l of ethanol was added in triplicate . The sample volume was 4.0 L. Some major biologically active compounds identified were Azulene (24.91%), Hexadecanoic acid (13.27%), . 2.3 GC -MS analysis The GC-MS analysis was carried out using a Clarus 500 Perkin- Elmer (Auto System XL) Gas Chromatograph equipped and coupled to a mass detector Turbo mass gold - Perking Elmer Turbomas 5.2 spectrometer with an Elite-1 (100% Dimethyl ply siloxane), 300 m x 0.25 mm x 1 m df capillary column. 10mg of the dried extract powder was encapsulated in 100 mg of KBr pellet, in order to prepare translucent sample disc. The presence of these compounds may proceed to find out various therapeutic activities. Hence GC-MS analysis was performed on methanol extract. The solution was boiled in a water bath and then incubated at 50-70C for an hour followed by filtering from What man paper [9]. are listed in the result. The present research was designated to investigate the methanolic extract used by GC-MS Technique (Thermo GC-Trace ultra version 5.0, Fisher, USA). The preliminary phytochemical screening reveals the . Conclusion: From this work, it can be concluded that the species contain eective phytochemical compounds, needs further research on toxicological aspects to develop a safe drug. (2005). The results concluded that the callus have more . The GC-MS analysis revealed the 18 chemical compounds. GC-MS analysis GC-MS analysis was carried out in a GC-MS: HP5890 fitted with a 1.4 m . Furthermore, GC MS analysis of the extract revealed the presence of 65 bioactive compounds. identied. Analysis was Electron ionization system was used; details of GC programme were given in Table 1. LC-MS/MS comparison of the 4 plant extracts imply that Y.aloifolia variegata L extract was the richest, which was apparent through its superior biological activity. 1: . Collection of Plant and Preparation of Extract Senna alata plant leaves were obtained from Oba Ile area of Akure and confirmed by Herb sellers at Oja Oba market in Akure. Gas chromatography-mass spectroscopy (GC-MS) is a combined analytical technique used to determine and identify compounds present in a plant sample [ 10 ]. The present investigation has helped to identify seventy five (75) bioactive chemical constituents from leaf, root in vivo and in vitro leaf callus methanolic extracts of Momordica cymbalaria by using GC-MS technique. them involve the use of plant extracts 1. peckinensis, cultivar; 'Winter pride') were planted by the direct seeding method in the greenhouse (Duksung Women's University, Seoul, Korea).Korean cabbages were grown for a 15 week growth period under an average temperature of 20~25C, 60~80% relative humidity and using natural fertilizers. Results: This investigation was carried out to determine the possible chemical components from P. chinense by GC-MS. Phytochemical screening of the sequential extracts of B.Cylindrica leaves revealed the presence of various bioactive components of which . The method of GC-MS analysis of seven different plant extracts (4 plants and 3 combinations) described here was developed by Wang et al. The GC-MS analysis of P. suberosa stem bark revealed the presence of twelve compounds (phytochemical constituents) that could contribute to the medicinal quality of the plant. Phytochemical screening of Trichosanthes anguina L. (Snake gourd) root was done through GC-MS . GC MS analysis GC-MS analysis was carried out on a Shimadzu GCMSQP2010 The Gas Chromatography-Mass Spectrometry (GCMS) analysis of the extracts was performed using a GC-MS (Model; QP 2010 series, Shimadzu, Tokyo, Japan) fitted withVF-5ms fused silica capillary column of 30 m length, 0.25 mm diameter, and film thickness: 0.25 mm. The GCMS analysis of ethanol extracts of both OPS and WPS samples were performed in GC-MS analysis of the extract was performed using a Perkin . The column used was Perkin Elmer Elite - 5 capillary column measuring 30m 0.25mm with a film thickness of 0.25mm composed of 95% Dimethyl polysiloxane. GC-MS analysis of extract of leaves Solanum torvum revealed the exsistance of 3-n-hexylthiolane, S,S-dioxide (49.407%) was found as the . GC-MS Analysis The GC-MS analysis of A.paniculata extracts was carried out on a Thermo GC 1300 and TSQ 8000 Triple Quadrupole GC- MS system with auto sampler Al 1310 at USIC Department of University of Rajasthan, Jaipur. The volatile compounds in the sample were analyzed by Gas Chromatography Mass Spectrometer (GC/MS) on an Agilent Robust methodologies aimed at efficient extraction of plant phenolics, their qualitative and quantitative analysis is desired. Leucophloea with the NIST & WILEY libraries, major Alkaloids are used as anaesthetic agents and are found bioactive chemical constituents found in the extract in medicinal plants [8]. GC-MS analysis GC-MS analysis were carried out . 0.02 of extract was dissolved in 1ml of glacial acetic acid and 1-2 drops of ferric chloride solution was added. GC-MS analysis GC-MS analysis of bioactive compounds in stem bark ethanolic extract of C. mala-elengi was performed at Kerala Forest Research Institute, Thrissur District, Kerala (Casuga et al., 2016). Further, the. The compounds were identified by comparison of GC-MS spectrum with library searches which led to identification of 18 compounds. The plant powder (10 gm) was taken and mixed with ethanol (150 ml).The mixture was boiled until and it was reduced . ZB 5- MS capillary standard Non-Polar column was used. The The 1.0 ml/min volume of methanolic extract sample injected into the instrument was The GC-MS analysis of ethyl acetate and methanol extracts from leaves, and methanol extract from rhizomes of A. nilgiricum detected the presence of 25 phytochemical compounds. gc/ms is a useful and dependable method for identifying complex plant extracts in an efficient and timely manner [53]. GC-MS Analysis: GC-MS analysis was carried out on a Perkin Elmer Turbo Mass Spectrophotometer (Norwalk, CTO6859, and USA) which includes a Perkin Elmer Auto sampler XLGC. 2.4 GC-MS analysis The phytochemicals of Kayea assamica leaf was analyzed by GC-MS. GC-MS analysis: The ethyl acetate plant extract of . 1L of the supernatant was injected into the GC and run for 30 minutes. GC -MS Analysis Gas chromatography Mass spectroscopy analysis of ethanolic extract was performed using Shimadzu GC-MS Model number: QP2010S equipped with Column - ELITE-5MS (30 meter length, 0.25 mm ID, and 0.25 m thicknesses). Pharmacog J, 9(1), pp. The extracts used for phytochemical tests were concentrated by evaporation and stored at 4C and used for GC-MS analysis. GC MS analysis of T. subulata plant leaf extract, . Researchers have explored extracts of plants including its derivatives for . The dry extracts were saponified with 2 mol/L KOH (dissolved in 50% ethanol) under reflux for 1.5 h. After cooling, the obtained extracts were separated in triplicate by liquid-liquid extraction with n-hexane. (iii) Gas Chromatography (GC-MS) analysis The GC-MS was performed by using Thermo GC- Trace Ultra Ver: 5.0, Thermo MS DSQ II. ISBN 978--521-56123-5. 4: Type of Chemical compound in Flowers extract of C. indica plant. GC-MS analysis: The Agilent 5975C Series GC/MS used in the analysis employed a fused silica column packed with DB-WAX (100 % dimethyl polysiloxane, 30 m 0.25 mm ID 2.5 m ). GC-MS procedure was performed by utilizing GC Shimadzu QP2010 framework and gas chromatograph interfaced to a Mass Spectrometer (GC-MS) outfitted with Elite-1 combined silica hair like segment. GC-MS Analysis of Ethanolic Extract of Alternanthera Philoxeroides and Alternanthera Bettzickiana from India U. Arul Pamila1 and S. Karpagam2 1PhD Research Scholar, 2Assistant Professor, Department of Botany, Queen Mary's College (A), Chennai-600 004. resulting extract was dried using a water bath to get semisolid. GC-MS analysis of phytocomponents on whole plant extracts Adiantum capillus-veneris L. - a potential folklore medicinal plant. GC-MS Analysis The crude ethanolic extract was dissolved in ethanol and votex mixed for 1 minutes and then centrifuged at 3000 rpm for 10 minutes. Figure 2 shows the mass spectra of methanolic extract of Moringa oleifera Fig 1 Shows GCMS chromatogram of methanolic extract of Moringa oleifera rotundus and C. vitiginea are analyzed and the phytoconstituents present are identified by GC-MS (Gas Chromatography-Mass Spectrometry) analysis. The analysis was carried out with the methanol extracts of the dried wild plant and callus of the three selected plants. the dried extract was stored in a deep freezer for further use. Plant extract with code Plant extract code Explanation R.D But Rosa damascena extract in butanol R.D Ethyl acet. GC-MS Analysis of Ethyl Acetate Extract of Whole plant of Rostellularia diffusa. Dimension 30Mts, ID: 0.25mm, Film: 0.25 m. Ebenezer T, Kadamban D, Shekhawat MS, Justin Packia J. It was . One study has stated that the mangosteen leaf extract was very effective against both, the Gram positive and the Gram negative bacteria, specifically those that were linked with skin infections like acne, diarrhoea, tuber- culosis etc. Fig. From the GC-MS analysis we concluded that chloroform extract has 15 constituents. CAS.N0 Retention Peak Molecular Structure Compound No. The prepared extract is also analysed by gas chromatography-mass spectrometry to identify and characterize the chemical compounds present in the extract. Flow rate of carrier gas was 1.2 ml/min. GC was performed using a Varian GC/MS 4000 equipped with a VF-5ms MS capillary column (30 m x 0.25 mm i.d., 0.25 m) (Agilent Technologies 7890A GC System, G3171A, USA). Preparation of plant extract Fresh plant was shade dried at room temperature for 10 days and powdered coarsely using electric blender. Phytochemicals such as . Fresh Korean cabbages (Brassica campestris L. ssp. GCMS chromatogram of the n-butanolic leaf extract of A.leiocarpus (Appendix 2) showed eleven peaks indicating the presence of eleven compounds. Fig. The screening of plant extracts is a new approach to find therapeutically active compounds in various plant species [1, 5]. Different extracts of targeted seeds showed different biologically active compounds that have different pharmacological potentialities. Identification was based on the molecular structure, molecular mass and calculated fragments. GC-MS Analysis GC-MS analysis was carried out on the ethanolic extract of Canthium parviflorum and 22 compounds were identified. Table 3: Analysis GC-MS of an extract of Leaves of C. indicza. In this study we performed GCMS, to analysis methanolic plant extracts of Momordica cymbalaria Fenzl. GC-MS analysis The GC-MS analysis of the extracts was performed using a Perkin Elmer GC-MS (Model Perkin Elmer Clarus 600) equipped with an . Nature of time (min) Area% Formula Name compounds 5 016526-56- 10.664 0.95 C 11 H 15 N 5 O 4 Adenosine, 2methyl 1 10 100037501-4 15.863 10.90 C 20 H . A brown ring at the interface indicated a deoxy sugar characteristic of cardenolides. Gas chromatography-mass spectrometry (GC-MS) analysis GC-MS analysis was carried out in a combined 7890A gas chromatograph system (Agilent 19091-433HP, USA) Several of these compounds are documented to . 30 m length). The sample of ethanolic extract was run for 35 minutes. It shows the presence of nearly forty constituents which includes flavonoids, 2. GC-MS analysis of the extract detected 50 compounds, most of which are found in same plant type of other country's origin. Investigations on the Availability of Bioactive Compounds in . Int J Drug Dev Res. 58. The major constituents were found to be 2-methylheptane (3.48%), cis-1,3-dimethylcyclohexane The crude extract form was subjected to column chromatography over silica gel (100-200 mesh) and eluted with nhexane, chloroform, ethanol and methanol- respectively. . The aim of the present study is to screen the phytochemicals present in the leaf extract of Bruguiera cylindrica (B.Cylindrica) and further analysis of the bioactive components present in it by Gas chromatography-Mass spectrometry (GC-MS) analysis. GC-MS chromatogram analysis of methanol leaf extract of mangosteen. The n-hexane fraction was evaporated to dryness and then used for the further analyses. GC-MS Analysis Gas Chromatography-Mass Spectrometry (GC-MS) is an analytical method that combines the features of Gas- Chromatography (GC) and Mass Spectrometry (MS). The GC-MS analysis shows different peaks with low and high molecular weight determining the presence of 41 phytochemical compounds.The Phytochemical compounds in the ethanolic extract ofLawsonia inermishave been screened by using GC-MS analysis. The electron ionization device used for GC-MS detection had a 70 eV ionisation energy. A small portion of the dry extracts was screened for the presence of chemical constituents like alkaloids, carbohydrates, glycosides, phenols, saponins, phytosterols, flavanoids, proteins and terpenoids [8, 9]. and GC-MS analysis of methanol extract of Ceropegia pusilla. GC- MS, with the use of internal standards, provides a multidimensional compound identification and quantification of the sample (Skoog et al., 2007). This family is one of the largest family in plant kingdom comprising of highest number of edible plant species. GCMS chromatogram of the methanolic extract of Moringa oleifera (Figure 1) showed eleven peaks which indicated the presence of eleven phytochemicals constituents. The carrier gas, helium, 70-72. subtilis 168 Isolated from . Kale MV. 17- the objective of this paper was to review and investigate the phytochemical constituents with gc-ms analysis and to establish the antibacterial property of the plant extract against the bacterial pathogens, escherichia coli and klebsiella pneumonia (which are proven causal organisms for all forms of urinary tract infections (uti) and also liver gc-ms chromatogram of the ethanol leaf extract of cassia alata (figure 2) showed 13 peaks indicating the presence of thirteen compounds with the retention time range between 2.72 and 34.54 (figure 2).the active principles in the methanol leaf extract of cassia alata were confirmed based on retention time (rt), molecular formula, molecular weight different solvent extracts of each plant material were used for FTIR analysis. Oven temperature was programmed to 60 oC for 10 min, and then increased at 20 oC/min to 230 oC and held at 250 oC for 10 min. n-Hexane extract of root of Rauvolfia serpentina was analyzed through GC-MS. Plant was later identified and authenticated at the Department of Crop Science, Federal University of by quantitative GC-MS analysis and phytochemical screening. The GC-MS analysis of R. humilis stem revealed the presence of various compounds which have been listed in table 1 and figure 1. and Research Department of Advanced Zoology and Biotechnology, Government Arts College for Men, Nandanam, Chennai, Tamil Nadu, India Antioxidant, antimicrobial activities and GCMS analysis of fruit extract of Solanum nigrum L. E ach of the extracts was analyzed by gas chromatography-mass spectrometry. Table 1. The objective of this present work is to identify the. spot obtained (Appendix 1) was used for GC- MS analysis. Results and Discussion The scientific validation and extraction of plant GCMS is one of the most precise methods to identify various secondary metabolites present in the plant extract The ethanolic extract of E. crassipes was analyzed by GCMS to detect various compounds with the help of NIST library. The present study is the comparative GC-MS analysis of an edible plant Alternanthera GC-MS analysis of the whole plant ethanol extract of P. chinense was performed using a Perkin-Elmer GC Clarus 500 system comprising an AOC-20i auto-sampler and a gas chromatograph interfaced to a mass spectrometer (GC-MS). International Journal of Advanced Scientific Research and Management , Volume 4 Issue 5, May 2019 www.ijasrm.com ISSN 2455-6378 GC-MS Analysis For Identification Of Bioactive Compounds In Crude Methanolic Extracts Of Leaves Of Origanum Majorana L. Plant Khushbo Bhardwaj1* and Widhi Dubey2 1 Faculty of Science, JECRC University, Jaipur, 303905, India 2 Faculty of Science, JECRC University . Methods Chemicals DOI: 10.1155/2022/5436476 Corpus ID: 251683465; GC-MS Analysis, Antibacterial and Antioxidant Potential of Ethyl Acetate Leaf Extract of Senna singueana (Delile) Grown in Kenya Generally it is used as a traditional medicine due to its medicinal values. Ailanthus triphysawere . extracts by GC-MS analysis. Students utilized QuEChERS to analyze spiked and incurred pesticide residues in several types of plant foods and applied GC-MS/SIM for the simultaneous quantitation . Plant materials preparations. GC-MS is one of the best techniques to identify the constituents in plants. GC/MS Analysis The chemical constituents identified by the GC/MS analysis of the methanolic plant extract of Mentha spicata (L.) was enumerated along with their molecular formula, retention time, and peak area (Table 3). The methanol fractions of each plant were taken for GC-MS analysis. The yield of their composition of extracts and essential oils differs due to different methodologies. 2012;4:148-53. and 7.35% saponins in plant sample. subtilis 168 Isolated from a River . The GC-MS analysis was done using the instrument GC-MS SHIMADZU QP2010 with GCMS solution version 2.53 software. Antimicrobial Activity and GC-MS Based Analysis of the Extract of Bacillus subtilis subsp. 0.5ml of concentrated sulfuric acid was slowly added. Carrier gas: He, Flow: 1.0 The program settings were capillary column TG-5MS AMINE (30 m 0.25 mm; film PDF | On Aug 25, 2022, Olasinbo Olumuyiwa Balogun and others published Antimicrobial Activity and GC-MS Based Analysis of the Extract of Bacillus subtilis subsp. The GC/MS analysis of Mentha spicata (L.) revealed the presence of 48 compounds identified in the methanolic extract. Then plant root extract (CBE) was prepared by mixing 1-5% of plant root powder with deionized water in a 250 ml of (Borosil, India) conical flask. The GCMS analysis of C. bicolor plant aqueous extract exhibited the presence of 98 compounds with 10 known bioactive uses. In the present work, compounds are identified by GC-MS of methanol extract of P.viscida and D. gangeticum, antibacterial The GC-MS analysis The GC-MS analysis of bioactive compounds from the different extracts of the leaves of B. luzonica was done using Agilent Technologies GC systems with GC-7890A/MS-5975C model (Agilent Technologies, Santa Clara, CA, USA) equipped with HP-5MS column (30 m in length 250 m in diameter 0.25 m in thickness of film). is study identified different classes of phytochemical constituents of. 9, 12-Octadecadienoic acid (ZZ) was the most potent bioactive compound present in three different extracts of both B. hispida and C . The identification of the phytochemical compounds was confirmed based on the peak area, retention time and molecular formula. International Journal of Research in Applied Natural and Social Science 2013; 1(3):49-58. Medicinal plants are rich in various bioactive compounds such as alkaloids, steroids, flavonoids, glycosides, from the 2-fold diluted plant extract stocks ranging from .4g/ml to 3g/ml were added in triplicate wells. GC-MS plays an essential role in the phytochemical analysis and chemotaxonomic studies of medicinal plants containing biologically active components [ 11 ]. Selvamangai C, Bhaskar A. GC-MS analysis of Phytocomponents in the Methanolic extract of Eupatorium triplinerve. The component identification was achieved by the GC-MS analysis using HP 6890 series GC (Hewlett-Packard. The methanol extract exhibited a better antibacterial activity than others. The medicinally important plants Cissus xavierensis, C. quadrangularis var. Helium was utilized as carrier gas. GC MS Analysis of Bioactive Compounds Present in Ethanol Extract of Combretum Hispidum (Laws) (Combretaceae) Leaves International Journal of Trend in Scientific Research and Development (IJTSRD) Volume 4 Issue 5, August 2020 Available Online: www.ijtsrd.com e-ISSN: 2456 -6470 mixed aqueous extract of D.gangeticum root is efficient in protecting the heart from ischemia reperfusion induced injury in diabetic rats 31 and the extracts of D.gangeticum have antimicrobial potential 32.

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