Angelo Vertti, 18 de setembro de 2022

A potentiometric whole cell biosensor based on immobilized marine bacterium, Pseudomonas carrageenovora producing -carrageenase and glycosulfatase A CaP coating on scaffolds is achieved by first precipitating amorphous CaP (ACP), followed by octa-CaP deposition, including a drug or bioactive agent of interest. Sort by Relevance. 115.71.239.220 Biomed. Values are mean SD. [18], The essential difference in the refining process is that the carrageenan is first dissolved and filtered to remove cell wall debris. Moreover, biomimetic co-precipitation-based -carrageenan functionalization of OCP might also improve precursor cell spreading as well as metabolic activity, which would provide a basis for further research on the suitability of -carrageenan functionalization of OCP coating on dental titanium implants. Yes [citation needed]. Innovative, high quality products for Genomics, Proteomics, Cell biology and Microbiology From manual to automated processes. We found that the addition of -carrageenan to the OCP coating slowed down protein (BSA) release during the first 2h of incubation, with the strongest inhibition at 2mg/ml. Healthc. A prominent advantage of using such an osteogenic agent/drug-containing CaP coating is that the biological activity of the agent/drug is preserved during coprecipitation with inorganic components at physiological temperature (37C) and pH (7.4) (Lin et al., 2020). Used under license. School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM Bangi, Selangor Darul Ehsan, Malaysia, Your session has expired. -carrageenan-functionalized OCP coating is attractive for osteoblast adhesion, and might be advantageous for osseointegration. (2020). -Carrageenan significantly reduced the pore size in the sheet-like CaP structure. (Camb.) (C) Calcium/phosphate ratio. There are two basic grades of carrageenan, refined (RC) and semi-refined (SRC). A study on phase separation behavior in kappa/iota carrageenan mixtures by micro DSC, rheological measurements and simulating water and cations migration between phases. doi:10.1111/cid.12050, Keywords: bone regeneration, -carrageenan, implant, OCP-coating, osteogenic differentiation, osteointegration, pre-osteoblast, titanium, Citation: Cao W, Jin J, Wu G, Bravenboer N, Helder MN, Schulten EAJM, Bacabac RG, Pathak JL and Klein-Nulend J (2022) Kappa-carrageenan-Functionalization of octacalcium phosphate-coated titanium Discs enhances pre-osteoblast behavior and osteogenic differentiation. Gene expression of all osteogenic markers (Runx2, Opn, Fgf2, Ocn, Mepe, and Dmp1) was similar on OCP-coated titanium discs without/with -carrageenan at day 1. Behav. In the current study, we functionalized the OCP coating on the surface of titanium discs by coprecipitation with -carrageenan. (2018). -Carrageenan in OCP coating on titanium discs modified the morphology and microstructure of the OCP-coating, and enhanced pre-osteoblast metabolic activity, proliferation, and osteogenic differentiation. 11, e2201248. Biomimetic CaP-based coatings on implants can serve as a vehicle for the targeted delivery of osteogenic agents, e.g., BMP2, to the local implant environment (Liu et al., 2018). FIGURE 9. Ocn is involved in calcium ion homeostasis and bone mineralization, as is Fgf2 (Globus et al., 1989). n = 9 from 3 independent experiments. Our data showed that -carrageenan-functionalized OCP coating on titanium discs robustly promoted pre-osteoblast spreading, as well as increased pre-osteoblast proliferation and metabolic activity, suggesting that -carrageenan-functionalized OCP coating is biocompatible and attractive to preosteoblasts and therefore likely to result in osseointegration and implant success. Browse Carrageenan and related products at MilliporeSigma. [1], Carrageenans contain 1540% ester-sulfate content, which makes them anionic polysaccharides. runt-related transcription factor 2 (Runx2), osteocalcin (Ocn), fibroblast growth factor 2 (Fgf2), dentin matrix protein 1 (Dmp1), and osteopontin (Opn) were determined. WebMenampilkan 298 produk untuk "caragenan" (1 - 60 dari 298) Urutkan: Paling Sesuai Ad Terlaris Carrageenan/karagen/karagenan pengenyal Rp227.000 Grosir Jakarta Utara Indo food chem 4.9 Terjual 3 rb+ Ad Karagenan / Kappa Carrageenan / Pengenyal Premium KRI-02 - 500 gram Rp73.900 Cashback 2% Surabaya CV. There are three types of commercially available carrageenans such as kappa ()-, iota ()- and lambda ()-carrageenan, which are differentiated according to the number and position of sulfate ester group attached to the disaccharide units, and are used to manufacture a wide variety of commercial products e.g. n = 3 from 3 independent experiments. The membrane has the hydrogen ionophore I immobilized therein by gel entrapment technique on a screen-printed Ag/AgCl electrode (SPE). it changed the more straight sheet-like structure of CaP into a more curved sheet-like structure. Carrageenan is an additive used to thicken, emulsify, and preserve foods and drinks. FIGURE 6. Implant Dent. Bar: 10m. A hybrid technology in which seaweed is treated heterogeneously as in the semirefined process exists, but alcohol or high salt levels are used to inhibit dissolution. Data were analyzed using Graphpad Prism 7.0 (GraphPad Software Inc., La Jolla, CA, United States). Bar: 5m. [16] The most commonly used sources are E. cottonii (Kappaphycus alvarezii, K. striatum) and E. spinosum (Eucheuma denticulatum), which together provide about three-quarters of the world production. Data curation, The selectivity of the P. carrageenovorabased biosensor towards -carrageenan was conducted based on SSM method by IUPAC, whereby the potentiometric responses of the microbial biosensor towards -carrageenan, -carrageenan and -carrageenan were evaluated separately in the concentration range of 1100 ppm. One-way analysis of variance (ANOVA) with Bonferronis post hoc test was used to test differences between groups. Webon kappa-carrageenan media as on agar media. CaP coatings on titanium implant surfaces have been widely investigated to integrate metallic implants with living bone (Das et al., 2019; Pazarceviren et al., 2021). 154, 606620. WebCAS: 11114-20-8 MDL: MFCD00151514 EINECS: 234-350-2 Sulphated plant polysaccharide. Department of Chemistry, Faculty of Science, Ibb University, Ibb, Republic of Yemen, Roles Cloudflare Ray ID: 7d12476d3967c091 Carrageenans are used commercially as thickeners and stabilising agents. Cell adhesion, spreading, and metabolic activity are directly correlated with osseointegration and implant success (Zamani et al., 2018). After reaching 85% confluency, cells were detached using 0.25% trypsin (Gibco) and 0.1% ethylenediaminetetraacetic acid (EDTA, Merck, Darmstadt, Germany) in phosphate buffered saline (PBS; Gibco) at 37C. Mat. doi:10.1002/jbm.820240306, Lee, N. K., Sowa, H., Hinoi, E., Ferron, M., Ahn, J. D., Confavreux, C., et al. This is likely due to the fact that -carrageenan contains abundant sulfate groups, which mimic the charged proteins present in the extracellular matrix and ensures the practicality of -carrageenan for bone tissue engineering. Bar: 15m. Therefore, -carrageenan is considered to be a potent factor that can induce bone regeneration. The number of bacterial colonies that formed on the surface of LB broth agar was calculated for each dilution, and the count lied between 30 and 300 colonies was selected to determine the number of bacterial colonies in bacterial colony-forming units per milliliter (CFU mL-1) unit. The pour plat method was used as a reference method using LB broth agar serial dilutions of bacterial suspension with tenth strength prepared in HEPES buffer. Brnemark, R., Brnemark, P. I., Rydevik, B., and Myers, R. R. (2001). -Carrageenan (0.1252mg/ml) increased the intensity of crystallinity peaks corresponding to the crystal structure of OCP, with -carrageenan at 2mg/ml showing the highest peak intensity. 12, 529534. https://doi.org/10.1371/journal.pone.0214580.g006, https://doi.org/10.1371/journal.pone.0214580.t003. Cultured bovine bone cells synthesize basic fibroblast growth factor and store it in their extracellular matrix. FIGURE 5. cDNA synthesis was performed using 0.51g total RNA in 20l reaction mix consisting of 5 units Transcriptor Reverse Transcriptases (Roche Diagnostics, Basel, Switzerland), 1mM of each dNTP (Invitrogen), 0.08 A260 units random primers (Roche Diagnostics), and 1x concentrated Transcriptor Reverse Transcriptase reaction buffer (Roche Diagnostics). The structure of -carrageenan is similar to glycosaminoglycan (GAG), which is naturally found in human bone and cartilage. Double (2) and triple (4) degenerate bending modes were observed at 427cm1 and 605cm1. Runx2 is a key transcription factor associated with bone-related cell differentiation. Cell lysate was collected using lysis buffer, and DNA content per well was determined with the Cyquant Cell Proliferation Assay (Molecular Probes, Eugene, OR, United States) according to the manufacturers protocol. Hydrogen ionophore I was incorporated in the poly(n-butyl acrylate) [poly(nBA)] as a pH ionophore. Integr. The medium was removed, cells were washed with PBS, and -MEM with 10% FBS and antibiotics was added. The developed potentiometric biosensor was then used for real sample study for recovery of a known amount of standard -carrageenan spiked into fresh milk. MC3T3-E1 pre-osteoblast proliferation was assessed by DNA content quantification. The physicochemical properties of the OCP coating without/with -carrageenan were characterized. Dose-dependent effect of -carrageenan in octacalcium phosphate (OCP)-coating of titanium discs on surface morphology. Bioeng. Mat. The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding authors. Osteoblast responses to injectable bone substitutes of kappa-carrageenan and nano hydroxyapatite. Avantor, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Mat. n = 3 from 3 independent experiments. Mat. Bulk and Prepack available at Sigmaaldrich.com. WebList of Kappa Sigma chapters. (F) Quantitive colorimetric results of ALP staining at day 7. One ROI was positioned in the center of each disc, and two ROIs were evenly spaced (200m) from the center of each disc in opposite directions. One of these natural polymers is -carrageenan, which is naturally available in high amounts, and easy to extract (Du et al., 2016). doi:10.1016/j.jmbbm.2015.02.005, Shen, X., Hu, W., Ping, L., Liu, C., Yao, L., Deng, Z., et al. (2021). Raman spectroscopy (DXR3, Thermo Fisher Scientific, Waltham, MA, United States) was used to identify functional groups, chemical interactions, and possible alteration of OCP coatings with -carrageenan. One ROI was positioned in the center of each disc, and two ROIs were evenly spaced (200m) from the center of each disc in opposite directions. Significant effect of -carrageenan, ****p < 0.0001. -Car, -carrageenan. Biointerphases 16, 020803. doi:10.1116/6.0000876, Pitaru, S., Kotev-Emeth, S., Noff, D., Kaffuler, S., and Savion, N. (1993). Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), LESTARI, Universiti Kebangsaan Malaysia, UKM Bangi, Selangor Darul Ehsan, Malaysia, Roles The biosensor exhibited a significantly higher response towards -carrageenan when compared with other types of carrageenan (p<0.001) and negligible responses were obtained from both - and -carrageenan over the concentration range of 1100 ppm, which suggests good selectivity of the potentiometric whole cell biosensor towards -carrageenan. undefined. Yes They have been mainly used for tissue engineering, wound coverage and drug delivery. 55, 8188. [32], As of 2018, carrageenan was deemed non-toxic under certain consumption levels (75mg/kg bw per day), although further research was recommended, mainly focused on the fate of carrageenan during and after digestion, and on any subsequent metabolites.[4][33]. All rights reserved. They can be mainly categorized into three different classes based on Buffer capacity was optimized by varying the PBS concentration between 1 mM and 50 mM, whilst the buffer pH was optimized between pH 4 and pH 10 by using 20 mM PBS. Future studies will test the stiffness, thickness, and stability of the coating, and address the underlying mechanism of -carrageenan-increased MC3T3-E1 cell spreading, proliferation, and osteogenic differentiation. A p-value <0.05 was considered statistically significant. [22] The European Food Safety Authority concluded "there is no evidence of any adverse effects in humans from exposure to food-grade carrageenan, or that exposure to degraded carrageenan from use of food-grade carrageenan is occurring",[23] Furthermore, the Joint FAO/WHO expert committee on food additives stated in a July 2014 review of carrageenan "that the use of carrageenan in infant formula or formula for special medical purposes at concentrations up to 1000 mg/L is not of concern". Small integrin binding ligand N -linked glycoprotein gene family expression in different cancers. These natural polymers are also used as bioactive interphases to enhance rapid, robust, and functional osseointegration between bone and implants (Caillol, 2020). 45, 166174. This work was also granted by Health-Holland (project no. Front. 13, 46. doi:10.3390/jfb13020046, Jin, J., Fei, D., Zhang, Y., and Wang, Q. FIGURE 3. Amorphous precursor-mediated calcium phosphate coatings with tunable microstructures for customized bone implants. Alkali treatment with NaOH allowed complete conversion of kappa () carrageenan form as detected by FTIR analysis. The concentration of buffer solution can affect the activity of P. carrageenovora. In this study, we present a simple potentiometric biosensor for direct detection of -carrageenan, being the most widely use carrageenan in the food industry as -carrageenan gel appears to be more firm than those obtained with - and -carrageenan. Biotechnol., 20 October 2022, View all The immobilized cell activity profile in different PBS concentrations at pH 7.0 is illustrated in Table 1. https://doi.org/10.1371/journal.pone.0214580.t001. SEM images revealed visual differences between MC3T3-E1 pre-osteoblasts spreading at OCP coating without and with -carrageenan (2mg/ml) at 1h after seeding (Figure 4A). 3 answers Aug 3, 2022 Hello everyone, I am going to prepare either an alginate or carrageenan hydrogels for immobilization of microalgae. Eng. The hydrogel membrane was then hydrated with 0.01 M HCl solution for 15 min. Exp. Thus, high concentrations of BMP2 released might promote the resorption of newly formed bone almost as soon as it has been laid down. Received: 04 August 2022; Accepted: 11 October 2022;Published: 20 October 2022. Mepe belongs to the small integrin-binding ligands and influences the adhesion and differentiation of cells from the osteoblast cell lineage leading to increased bone formation (Sprowson et al., 2008). (A) Alizarin red-stained mineralized matrix. (E) ALP protein staining at day 7. All the solutions were prepared in Milli-Q deionized water (18 MOhm). A strong band at 961cm1 was assigned to the totally symmetric stretching mode (1) of PO43 group in OCP. Conceptualization, WC, GW, JK-N; Formal analysis, WC, JJ; Funding acquisition, WC, JP, JK-N; Investigation, WC, JJ; Supervision GW, NB, MH, ES, JP, JK-N; Writing-original draft preparation WC, JP, JK-N; Writing-review and editing, JJ, GW, NB, MH, ES, RB, JP, JK-N. All authors have read and agreed to the published version of the manuscript. Biomaterials 25, 20192027. Relationship between osteopontin and bone mineral density. LSHM19016, BB). Kappa-carrageenan is a relatively cheap support carrier, which can substantially reduce costs in a large-scale production. However, the practical application of the polymeric gel carriers including -carrageenan beads has been limited by the problems of gel degradation and low mechanical strength. Interfaces 6, 31323140. Dose-dependent effect of -carrageenan in OCP-coating of titanium discs on extracellular matrix mineralization in MC3T3-E1 pre-osteoblasts at day 21. The fraternity was established at the University of Virginia in The optical path is 10 mm. Quickly find the specific antibody you need from VWR's broad portfolio We are here to help you improve efficiencies and outcomes in your laboratory. Clin. 17, 509516. To compare product details, select up to 3 alternatives below and click Compare Selected. Values are mean SD. At the same time, the latter enzyme catalyzes the enzymatic desulfation of neocarrabiose sulfate to neoagarobiose [3-O-(3,6-anhydro--L-galactopyranosyl)-D-galactopyranose], sulfate and hydrogen (H+) ions [25, 3435]. OCP coating with -carrageenan (2mg/ml) Increased mRNA levels of Runx2 (2.94-fold), Opn (3.59-fold), Fgf2 (3.47-fold), OCn (3.88-fold), and Dmp1 (4.59-fold) compared to OCP-coated titanium discs without -carrageenan at day 21. Furthermore, the biosensor based on disposable SPE is low-cost and provides portable version of test device for on-site routine analysis to be carried out in a convenient manner. (2014). milk products, processed meats, infant formula, toothpaste, cosmetics and pesticides [6]. A double junction Ag/AgCl reference electrode and the bacterial cells-modified SPE working electrode were connected to an ion meter (Orion) for potential (electromotive force) measurement. Collagen-hydroxyapatite/-carrageenan (COL-HAP/-Car) composite has been shown to have similar structural characteristics with natural bone (Feng et al., 2017). Tools and services to accelerate your research & development. Chem. Language links are at the top of the page across from the title. In this study, -carrageenan did affect Mepe gene expression at 14days, but not at 21days, suggesting that -carrageenan affects Mepe expression only in the short term. for carrageenan was developed in the Philippines. doi:10.2147/ijn.s193176, Kim, H. D., Amirthalingam, S., Kim, S. L., Lee, S. S., Rangasamy, J., and Hwang, N. S. (2017). Therefore, carrageenan continues to meet the OFPA criteria for inclusion on the National List. Copyright 2022 Cao, Jin, Wu, Bravenboer, Helder, Schulten, Bacabac, Pathak and Klein-Nulend. https://doi.org/10.1371/journal.pone.0214580.t004. Solutions for your entire workflow from basic research to biopharma production. Find the biological materials you need for upstream bioprocessing, including bulk-sized biological buffers and biochemicals Avantor supports end-to-end fluid management solutions including Masterflex peristaltic pumps and aseptic fluid transfer solutions that are reliable and customer-centric, helping bioprocessing manufacturers meet their research and production goals. There are three types of industrial processing: This is only produced from E. cottonii or E. spinosum. The membranes were prepared by a solution casting method. The -c solutions were prepared by dissolving -c in ultrapure The SPE-based biosensor gave satisfactory relative standard deviation (RSD) at 2.6% for three consecutive -carrageenan measurements with three different biosensor electrodes indicating high reproducibility of the sensing elements for measurement of -carrageenan. FIGURE 4. doi:10.1016/j.actbio.2018.10.023, Haugen, H. J., and Chen, H. (2022). (2020). The immobilized P. carrageenovora concentration was studied to determine the optimum bacterial cell concentration for generating maximum potentiometric response. Values are mean SD. Carrageenans are high molecular weight sulfated polysaccharides made up of repeating D-galactose residues and 3,6-anhydrogalactose, which are linked via alternating -(1,3) and -(1,4) linkages [12]. The surface morphology of OCP-coated discs without/with -carrageenan was observed by scanning electron microscopy (SEM; S-3400N II, Hitachi, Tokyo, Japan). -Carrageenan was kindly supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), and was prepared as described earlier (Du et al., 2016). The latter was detectable by H+ ion transducer for indirect potentiometric quantification of -carrageenan concentration. FIGURE 2. Con, control; -Car, -carrageenan. At day 7, -carrageenan (2mg/ml) increased ALP protein by 1.49-fold (Figures 7E,F).

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