Angelo Vertti, 18 de setembro de 2022

(yes, there are a lot on this page, and George gives even more, so what? Then add later (which is tedious in LAS X). This enables much simpler quantitation of fluorescence signals than conventional PMTs whose digitizers output values that depend on both the High Voltage ("HV") gain and the "offset". Nathan Shaner's 6/2019 bioRxiv preprint on AausFP1 bright new GFP could be excited precisely at excitation maximum, AOBS emission side precisely cover emission peak and same for the YFP varient). I recommend saving with your name, data, specimen number (ex: MaryEG 20210302 slide01). * We are very open to labs purchasing -- in exchange for time credit -- additional components for the Leica SP8 microscope. Leica LAS X Navigator and TCS SP8 FALCON Tandem scanners (select at software initialization). I estimate 10x advantage for each 3rd gen HyD at less than 2x the cost of each2nd gen HyD. I note we would need a lot of user hours to pay for both annual service contract and buy 3rd gen HyDs from the daytime hourly rate of $27/hr) could provide money to (i) buy one or more of the 3rd gen HyDs. Long term, the SP8 scanhead does not have an X1 port -- this is upgradable in the field. College of Staten Island Foundation, Inc. In addition, if your research needs to change, the system can also be adapted or upgraded at any time. With White light laser ("WLL", Leica typically offers 440-900 nm excitation) and NKT Photonics "EXTEND-UV" to get down to 350nm excitation, STELLARIS/WLL/EXTEND-UV/POWER5internal/POWERX4/fast computinginfrastructure, would be amazing. The scheduling will move to iLab when the ACCM's iLab page is activated. The Infinity Port Connector, along with complete optomechanical design documentation, opens the Leica DMi8 light path to any accessory you want to add. Faster speeds require zooming. Leica Microsystems allowing you to get up to four times more field of view (35 mm in one glance) compared to standard overview objectives. January 31, 2019: Ross S910 room hosting the ACCM Leica SP8 confocal microscope has been renovated with (i) blackout curtains for each microscope station, (ii) three pairs of track lights for each station, railings for the air ducts for HEPA filters. So: transfer to your own space ASAP (i.e. We hosted SP8 FALCON demo August 2018. Every DMi8 comes standard with 19mm field of view (FOV) for all camera ports. Overview. confocal microscope resolution equation (1.0 Airy unit standard of care); dxy = 0.51 * Lambda / NA Nyquist theorem (2D) suggests ~3x sampling for pixel size. I note that Alexa Fluor PLUS secondary antibodies from ThermoFisher are 3x brighter, though 1.3x more expensive than plain AF antibodies. Scan speed (usually use 600 Hz at 1x zoom) this and image dimensions (below) control pixel dwell time = amount of time each pixel is looked at. * we also have printouts on the Leica table (and nicer formatting). We have 4 lasers: 405, 488, 552, 638 nm. Define both digital and analog signals, and set up the trigger signaling independently from the image acquisition with exact timings and full reproducibility. In May 2020 leica introduced new STELLARIS Confocal microscope (I think of it as 'SP11' since prior numbering was SP2, SP5, SP8). Change from the macro (35 mm) to nano (200 nm) with only a click. Common 2*2 immunofluorescence experiment: scan track 1: 488 nm and 638 nm lasers, AF488 on HyD1, AF647 on HyD3. Motorized XY scanning stage (control by remotes or software, do not touch the yellow motors). If dim signal: 16 line accumulation and 'whatever needed' frame accumulation you need to get good data (overnight or over weekend if necessary though you should think about better fluorophores, more laser power, tyramide signal amplification, etc, to get better signal). FRAP/FLIP, FRET; Microscopes: Leica DMi8 CEL Advanced (inverted) Lasers: 405 nm; 488 nm; 552 nm; Conventional fluorescence filters: Analyzer; Dapi; Green; Red; Objectives: HC PL FLUOTAR 10x/0.30; HC PL APO 20x/0.75 CS2; HC PL APO 63x/1.30 GLYC CORR CS2; HC PL APO 40x/1.25-0. All our pre-owned Microscopes Confocal include a worry-free 180-day warranty. A fixed 405nm violet laser and pulsed white-light laser (WLL) that is tunable from 440-790nm allows for precise excitation control and multi-spectral imaging. The Hamamatsu Flash 4.0 sCMOS camera guarantees fast image acquisition with a large field of view (2048 x 2048 . If you use #0, ~100 um thick, you can get an additional ~70 um working distance, with only modest loss of image quality from standard coverglass. Example, 500-540 nm is away from 488 and 552 nm laser lines. Load your settings (top of HyVolution2 controls; please turn off Z-series and timelapse=1, prefer Z position down all the way, before saving settings). Enable "Calculate a Point Spread Function" to have the web page compute and display XY and XZ images of point spread. Confocal zoom range 0.75 - 48.0, so 63x lens is effective 42x - 3024x (not that anyone will benefit from latter zoom). open menu ISU Advanced Bioimaging Facility ISU Leica SP8 Specifications ISU Leica SP8 Gallery Videos ISU Leica SP8 Specifications ISU Leica SP8 Confocal System/White Light Laser/Falcon Microscope: -DMi8 CS inverted microscope; DIC -Air Table / Compressor -Super Z Galvo Stage; optional Universal or Multiwell plate inserts Find company research, competitor information, contact details & financial data for SARL DEBRUYNE of LAMBERSART, HAUTS DE FRANCE. ZERO BIAS - scores, article reviews, protocol conditions and more Total pixel dwell time (TPDT): product of line accumulation (ex. 10x objective lens Its billing is in hour intervals, rounded up. If you have coverglass-slide or 35 mm imaging dish, typically your specimen should look completely transparent (or nearly transparent if thin tissue section or tissue culture cells). New user training notes (see also New User Training QA QC SOP doc): We normally train new users in two 2 hour sessions, ideally consecutive days. CMN Core has a new Leica Stellaris 8 Confocal microscope. guiDeD WorkFLoW: high-enD MicroScopY For everYBoDY the Leica DMi8 makes it easy to achieve accurate results - for everybody on your team. To me 3x brighter is a win, since you might have $3 vs $4 in antibodies vs $27/hr confocal time. And because the objectives are positioned below the stage the risk of collision with the sample is reduced. GM configures settings, evaluates crosstalk, adjusts any settings if needed, explaining to user, saves settings which user can load for future sessions. No messes! This would enable "fast photon counting" with the four SMD HyD's (8x faster counting than now). The Facility features a 2019 Leica SP8 Confocal Microscope with White Light Laser and Fluorescence Lifetime Imaging Microscopy (FLIM) capability. I note that SMD HyD's are also FLIM (fluorescence lifetime imaging microscopy) capable, so if a FLIM compliant pulsed laser (ex. Personalize the function keys to your preference and use coded components and the illumination control. the Leica SP8 scanhead can use four HyDs,so ideally buy FOURnew 3rd gen HyDs and hopefully get Leica to accept the two current 2nd gen in trade-in). GM note: standard coverglass is #1.5, ~170 um thick. the Leica "laser box" can accept one more laser line (695 nm, possibly ~350 nm). The Leica SP8 confocal microscopeis on a Leica DMi8 inverted microscope stand (DMi8CEL). Specifications:Dimensions: (WxDxH) 335 x 100 x 175 cm (11 x 3.3 x 5.9 in. * Having 3 epi-illumination detectors (2 HyD, one conventional) means maximum 3 fluorescence channels per excitation "scan track". We would especially like to see 13plex acquisition per laser line + multiple laser lines + "joint" spatial deconvolution and spectral unmixing, optioanally spectral phasors, per: Hoppe (2008 and 2016), Valm&Borisy (120plex 2016), Fraser ('spectral phasors' 2017) groups publications on parts of these. If you logged into myJHU and/or MyJHU->Microsoft OneDrive, sign out of each. June 13, 2018:Leica SP8 is now in Ross S910A(S = Service corridor). 100 to 300 300 needs to use 16-bit mode [12-bit would work, but the image file is going to be 16-bit inside LAS X and export to raw TIFF, so might as well use 16-bit format). I simplify toconfocal sweetest spot 0.66Airy unit. GPU-based navigation, FLIM and Super-resolution, APPLICATION TALKS - June 19, 2018, 12:00-1:15PM Call. so we choose to not provde recommendations for this lens. Combination is rated for 140 nm XY resolution (acquire with 50nm or 40nm pixel size, typically Z = 3 * XY pixel size, so 150 or 120nm), compared to "conventional" confocal ~210 nm XY resolution (at 500 nm emission wavelength 405 nm excitation of any of BUV395, BV421, SB436 may enable better resolution). We have a NAVIGATOR license. Tip: If you use immersion oil, clean off your slide with a dry kimwipe(s). 10) * frame accumulation (ex. See also their "Explanation: proper images" and additional text on their web page. 610-721-7133. This laser based confocal system permits measurements of fluorescently labeled tissue sections, individual cells, or particles adhered to matrices over a wide selection of visible wavelengths. In addition, see more through the eyepieces with up to 25 mm FOV. Microscope Platform (Leica DMI8 CS, 2023) Fully motorized stage for optimized for tiling and navigation; Super Galvo Z stage (500 nm movement . See more of your sample in a shorter time thanks to the Macro* objective offered exclusively by Leica Microsystems. Zeiss Stereology and Neurite Tracing . The PC is for image acquisition not surfing the web or dark web, etc (looking for spectra, imaging product data sheets, is ok). We have "HyVolution2" = Leica SP8 confocal and Huygens Essential. "super-duper resolution" resolution (1.414x improvement): 0.3Airy unit pinhole size, 40 nm XY pixel size, 120 nm Z-step size. * the Leica SP8 confocal microscope does not currently have an environmental control incubator system. * Jeff Reese, NIDDK / NIH, wrote in Confocal Listserv wrote they like "0.6 - 0.7 Airy unit as sweet spot" for confocal. Trick for SP8(eventually hopefully Leica will publish a user generated application note I wrote about this): You can double the acquisition rate by using both HyDs across the spectrum of one fluorophore, and then ADD the two channels in LAS X (or ImageJ or MetaMorph, etc). Example: hemacytometer coverglass is 400 um thick (we measured the thickness 'edge on' by standing the coverglass on edge 'mind the gap' the hemacytometer counting gap height is 100 um). The instrument is equipped with 4 air cooled lasers allowing for excitation wavelengths of 405, 488, 552 and 638 nm. Once you know what you are doing, you could acquire "over lunch" (or over long lab meeting), or overnight (or over weekend), unattended -- and use your time better that confocal sitting if you do not need to be one hand. * $20/hour for fully trainedexpertusers, outside JHMI business hours (outside Mon-Fri 9am-5pm). FALCON - HANDS ON DEMONSTRATION TIME -August 13-16 (Mon - Wed) In practice; good luck getting anywhee close to that resolution on biological specimens and in a building that vibrates (all buildings do). The Leica Stellaris 8 is the most modern and fully featured laser scanning confocal microscope offered by Leica. MicFac -- that have found money for super-resolution. Flexibility is built-in, allowing you to add established options like DIC for unstained samples and Intelligent Automation. Invest in the features you need for your work now and be prepared for future requirements. Smaller pinhole sizes expected to reduce light level -- by how much is NOT simply proprotional to relative pinhole area: best to measure for yourself. Confocal 0.66 Airy Unit at 430nm fluorescence emission: dxy = 0.94* 0.51 * Lambda / NA =0.51*430nm / 1.3 = 148 nm that is, I estimate ~6% improvement in resolution at 0.66 Airy unit. Brilliant Violet 421 = BV421, also the "421 component" of the many BV tandems (2 channels). Environmental Enclosure with heat and humidity. From basic documentation to advanced life science research LAS X directly navigates you to brilliant imaging! Useful; for looking. For time-lapse experiments use precise focus control with Adaptive Focus Control and Closed Loop Focus. Microscopy. Scanner with Scanfield Rotation See more details of your sample in less time. Ideally purchase FOUR SMD HyD's and negotiate trade-in of the current two 2nd gen HyD's. GM recommends for growing cells in Mattek or similar 35 mm imaging dishes (#1.5 or precision 170 um coverglass bottom), using minimal amounts of expensive reagents for labeling (antibodies, single molecule RNA FISH), large volume (2 mL) for inexpensive reagent wash steps. Fully configurable with manual to motorized components, you can create the imaging system for your research and budget needs. POWER HyD(TM) are as fast or faster than 3rd gen HyD, so in fast FLIM mode, a whole lotta data (which 2020 computers can deal with: E-ATX motherboard, PCIE gen4, NVidia Ampere GPUs, 1+ Terabyte fast RAM, lots of PCIe gen 4 NVMe SSD drives (re ASUS Hyper M2 or GloTrends cards), 10Gbe Ethernet (maybe 40Gbe) to local 'distributed computing network'. See the award page for more details. HyD detector(s): photon counting mode please! 63x / 1.40 NA (oil immersion) pinhole 1.0 Airy Unit, 63x / 1.40 NA (oil immersion) pinhole 0.666 Airy Unit. An inverted wide field microscope. Lightning (adaptive deconvolution) Summary of Instrument Features: Microscope: Inverted Leica DMi8 Microscope Software: LAX Software Objectives: Standard: 93x/1.30 - Glyc - HC PL Apochromat C52 63x/1.40 - Oil - HC PL Apochromat C52 20x/0.75 . Do NOT close either of the shutters (if either shutter was closed at the beginning of your session, open both. Normally LAS will be running and HyVolution2 mode already selected. The new DMi8 S platform extends the flexibility of the DMi8 microscope, adding high speed control, Infinity TIRF and Infinity Scanner modules, plus advanced software capabilities to create the solution for your advanced live cell imaging. Lasers: 405 nm diode, Argon with 458, 476, 488, 496 and 514 nm lines and a White Light Laser with spectral emission from 470-670 nm. 500-540nm with adjacent 8nm bands; no need to waste any of the five internal detector positions with a PMT like our SP8 /// I also note that the STELLARIS external X1 port could be configured to be used with POWER detectors, would be great to have say 4 more POWER detectors externally for nine total [or even more with more money). Talk to our experts. (919) 660.1234, Medical Sciences Research Building III Cluster, Andor XD Spinning Disk Confocal Microscope Manual, Andor Dragonfly Spinning Disk Confocal Microscope (User's Manual), DeltaVision Elite Deconvolution Microscope, Leica AM Total Internal Reflection Fluorescence Manual, Live Cell Station (Zeiss Axio Observer Z1) Manual - With MetaMorph, Live Cell Station (Zeiss Axio Observer Z1) Manual - Single or Tile Imaging With ZEN, Olympus VivaView FL Incubator Microscope Manual, ZEN 2.3 Pro: Single Field or Tiling/Stitching Multiple Fields, Zeiss Axio Imager Z2 Upright Microscope Manual, Zeiss MosaiX - Color brightfield tiling and image stitching on the LCM, Zeiss PALM MicroBeam Laser Capture Microdissection Manual, Paraformaldehyde, Formadehyde and Formalin, Stimulated Emission Depletion super-resolution imaging, High sensitivity point scanning confocal with pulsed white light laser, High-sensitivityGaAsPHyDdetectors with gating and photon counting, 3D z-stacks,timelapse, stitching, multi-positiontimelapse, STED depletion lasers: 592nm, 660nm, 775nm, Resonant scanner (8 000 Hz) - 28 fps at 512*512, Green (I3 = xBP470-490, dichroic 510, mLP515), Red (N2.1 = x515-560, dichroic 580, mLP590, 20x NA 0.75 HC PL APO multi-Imm Corr (oil, water, glycerol) DIC WD 270 um, 40x NA 1.3 oil HC PL APO CS2 OIL DIC WD 240 um, 93x NA 1.3 glycerol with motorized correction collar (for STED) WD 0.3mm, Five channels - two PMTs, three HyDs - high sensitivity Hybrid Detectors - PMT1 | HyD2 | PMT3 | HyD4 | HyD5, All spectral detectors; HyDs have gating for STED, Huygens deconvolution software linked to LAS X for STED deconvolution. We encourage evaluating using "max speed" 1800 Hz with summing the HyD photon counts (line accumulation = 10, for example). Second session GM walks user through loading settings, reminding safety features (make sure objective lens will not crash into specimen or holder). When you change the contrast method, the microscope automatically adapts the illumination settings, parfocality, brightness, and diaphragm position to that method. It was funded by a Research and Revitalization Program Grant from the UW Madison OVCRGE. fromhttps://downloads.leica-microsystems.com/Leica%20TCS%20SP8/Brochures/Leica%20TCS%20SP8%20Scan%20Head-Flyer_EN.pdf. For example, 1800 Hz requires zoom 7.5x or more (you control number of pixels). * Four laser lines: 405, 488, 552, 638 nm. I note that MetaMorph can open 16-bit TIFF (and can open an image directly from LAS X's .LIF container file) but MetaMorph does not read HDF5 and does not deal with 32-bit data. In addition, the Leica TCS SP8 features 3 HyDs (hybrid detectors combining the characteristics of a classic PMT with a highly sensitive avalanche photodiode) and 1 PMT which can measure real time response of time-critical sequences due to its fast scan speed. Combine high speed External Filter Wheels with multi-position stage experiments and benefit from filter wheels with high precision stage control. Leica Workshops hosted by Ross Fluorescence Image Core and ACCM Confocal Microscope Core. June 1,2018: (see also above) Leica SP8 calendar/scheduler moved from Ross FIC (this site)to iLab Organizer as ofJune 1, 2018 (this scheduler booked all the time or calendar removed).***. 1000x1000 pixels vs 2000x2000 pixels). Microscope Details. The DMi8 modular research microscope is the heart of the DMi8 S system. will be billed a block of time each month (ex. For standard applications, use the easy to install filter cubes with automatic RFID identification. Leica Z-galvo and motorized XY stage. The new confocal microscope can use the 3rd gen HyD, or an NIR version (i.e. prior user got oil on the 20x lens), contact George immediately (email above; Ross 913 office [903 during covid-19 space utilization reduction), 305-764-2081 cell) George cleans the lenses. ==> Dr. McNamara reviews sessions on the sign-in sheet when doing the 'confirm' step in iLab Organizer. *STELLARIS 5 CW and / or pulsed lasers, optional white light laser (WLL) laser (~480 - ~700nm, see data sheet) and AOBS, TauSense "fast FLIM" (fluorescence lifetime imaging microscopy). The final results are razor-sharp images taken at the perfect moment, even when acquiring with maximum speed. Huygens Essential deconvolution (SVI Hyugens Essential intregrated into Leica LAS X software). : Regular updates and upgrades of LAS ensure that you are always ahead of the game. If you image through a 400 um thick object object that is R.I. 1.600, the 'apparent Z' is 250 um that is, the Z-motor will travel 250 um. The Department of Anesthesiology & Critical Care Medicine (ACCM) users are aiming to use 20 hours per week, that is,50% of JHU business hours access (it's ok if ACCM uses more). Image dimensions (ex. Leica Microsystems exclusively offers Ultra-Contrast 3D illumination. The Leica SP8 confocal microscope is on a Leica DMi8 inverted microscope stand (DMi8CEL). Like a GPS for your cells, the software moduleLAS X Navigatorensures that you always have a clear roadmap to brilliant data. The SP8 is funded by a grant from the National Science Foundation. * STELLARIS 8 CW and / or pulsed lasers, optional white light laser (WLL) laser (~440 - ~780nm, see data sheet)and AOBS, TauSense "fast FLIM" (fluorescence lifetime imaging microscopy)AND FALCON"fluorescence lifetime contrast" (fast FLIM, Phasors, other fancy FLIM stuff). See the Videos page for a discussion of the facility and some of the available applications. If you need super-resolution (beyonf standard of care confocal 1.0 or 0.66 Airy unit), consider using one of the other microscope facilities on campus -- i.e. June 1, 2018 was the start of the iLabs Organizer reservations for our Leica SP8 confocal microscope (much more efficient than the old reservation and billing system): If a user needs their PI or administrator to set up account numbers (IO#'s in JHU accounting jargon), see 1) * pixel dwell time. Our HyD's are "2nd generation", in mid-2018 Leica introduced single molecule detection SMD HyD's, that each count 2x faster than our 2nd gen HyD's. Have a Tokai Hit for CO2 incubation and temperature regulation. Pixel size (I train users to start with resolution limit, such as 120 nm for the 20x objective lens with confocal pinhole 1.0 airy Units (the standard of care for confocal); larger pixel size gives less detail and more signal, up to the limit of the laser spot size; smaller pixel than resolution limit just results in longer scan time, bigger data sets with special settings, such as 0.5 Airy unit pinhole, could get about 20% better resolution but only the amount of photons, which is not usually a useful trade-off). No matter if you work in metallography, medical device manufacturing or microelectronics, speed is essential. APDs are 70% quantum efficiency in the red and near infrared (~600 - 900 nm emission), which would enable further multiplexing, discussed at: https://www.linkedin.com/pulse/resolution-blues-meets-21plex-salute-fluorescence-basic-mcnamara, https://www.linkedin.com/pulse/bd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara. Intracellular distribution of fluorescence can also be quantified by way of 3D imaging. GM then shows how to acquire Z-series (if user project needs most do). There is an channel unmixing "matrix" to let you unmix 'spectral overlaps'. That is POWER HyD(TM) is NOT a PMT-APD hybrid detector, but something else. Do you need to work with big samples and analyse more of them? January 5, 2018 news: ACCM's image core will be activated on iLab Organizer. The Leica Application Suite (LAS) software lightens your workload by offering a variety of expert modules, e.g. GM thinking: we would love to have a STELLARIS 8 confocal microscope here. Location: Ernest Mario School of Pharmacy, William Levine Hall, Room 002. The new DMi8 S platform extends the flexibility of the DMi8 microscope, adding high speed control, Infinity TIRF and Infinity Scanner modules, plus advanced software capabilities to create the solution for your advanced live cell imaging. 617-253-6403, 2023 Massachusetts Institute of Technology, DeltaVision Ultimate Focus Microscope with TIRF Module, DeltaVision-OMX Super-Resolution Microscope, Nikon Spinning-Disk Confocal Microscope with TIRF module, Nikon A1R Ultra-Fast Spectral Scanning Confocal Microscope, Olympus FV1000 Multiphoton Laser Scanning Confocal Microscope, Olympus FV1200 Laser Scanning Confocal Microscope, Arcturus Laser Capture Microdissection system, Preparing ES cells for Blastocyst Injection, Koch Institute for Integrative Cancer Research, Leica DMi8 microscope with 5X, 10X air, 25X water, 63X and 100X oil objectives. Do you prefer personal consulting? Create fast overviews of your samples and identify the important details instantly. A cool feature of STELLARIS confocal scan head is it can use FIVE (!!!!!) For routine to live cell research, the DMi8 S platform is a complete solution. Benefit from the cost-effective manual version of the Leica DMi8. Specifications. Used kimwipes, paper towels, lens paper, goes in the biosafety trash immediately. Huygens HyVolution2 (see above) -- can improve resolution by ~10%, depending on a lot of things, including perfect specimen preparation (as a user, are you perfect? All lasers to stand by Very nice for low mag confocal imaging. We are happy to answer all your questions and concerns. The modular DMi8 inverted microscope is the heart of the DMi8 S platform solution. Every DMi8 microscope can be equipped with up to two Infinity Ports, allowing direct access to the infinity space for flexible upgrading of your DMi8. This trick will be much more interesting with Leica STELLARIS 5 and 8 (launched May 2020: we would welcome upgrade!) It allows you to illuminate your sample from different angles, obtain additional information on the surface structure, and achieve improved contrast. Location TBD Contact Dane Jensen ( ddj3@nyu.edu) Imagine a world where everyone smiles Flow Cytometry/Cell Sorting & Confocal Microscopy. If you get oil on the 20x lens, or it looks hazy compared to the 20x lens (i.e. Then set up high resolution image acquisition automatically using templates for slides, dishes and multiwell plates. Confocal microscopy was carried out using a Leica DMi8 confocal microscope. For more information or to reserve a demonstration time, Leica DMi8 A System, Sample Preparation for Electron Microscopy. If you have money, and especially if you need speed and/or plex, please consider purchasing SMD HyD's for this SP8 confocal microscope. Leica DMi8 M / C / A Inverted Microscopes for Industry Invert The Game - And Stay Ahead Being ahead of the competition is what drives your business. Has user drive as much as possible. Make every component work in harmony, at the highest speed possible. Combine Leica optical quality, a wide range of contrast modes, and intuitive software in one system to help you speed up your workflow. Lasers: 405 nm diode, Argon with 458, 476, 488, 496 and 514 nm lines and a White Light Laser with spectral emission from 470-670 nm. "Rab7 and . NYU.edu requires JavaScript be enabled in your browser in order to use important features of the site. See thehigh speed acquistion of an entire slide. All three models can have X1 port with additional detectors, such as two S dtectors or four APDs (avalanche photodiodes, which have ~80% quantum efficiency in red-NIR but slower photon counting and dynamic range per detector than S detector). Please note that Z-step size is the physical amount the Z-motor moves the objective lens. For specialized live cell applications, you can now add additional devices to the system and set up high-speed experiments with trigger controled third party devices. The DMi8 S imaging solution powered by LAS X Synapse advanced sequencer eliminates the bottlenecks between system components, resulting in dramatically faster imaging. The "photon counting speedadvantage" of 3rd gen HyD's vs current 2nd gen is non-trivial to figure out under real use conditions. Configure your system with a variety of contrast method, add sophisticated modalities like TIRF. Frame accumulation number of times each frame is scanned I usually recommend starting with 1 (max is 16). If you get 'significant' assistance mage core staff: We thank George McNamara, Ph.D. for assistance with the ACCM confocal microscope (routine training and question answering does not rate acknowledgement). Hardware zoom 0.75x - 48x (by changing confocal scanning area with the XY scanning mirrors). The instrument has a maximum image resolution of 64 Mpixels. There is limited space, so please email kaedwar@ilstu.edu if you plan to enroll. 440 nm 80 MHz, or fiber laser) was purchased, could do "fast FLIM" (would also need upgrade of LAS X software to LAS FALCON fluorescence lifetime contrast). (ii) if non-ACCM users add up to more than 20 hours in any work week and ACCM users did not need 20 hours: great for everyone.

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