maxima sybr green qpcr master mix
1 mL ROX solution, 50 M 2 x 30 mL Nuclease-Free Water Store at -20C. The ultimate result must be no CT in NTC and early CT in Positive sample . Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. of 25 l . SYBR Green/Fluorescein qPCR Master Mix (2X)-K0243-4000 reactMilliporeNEB Raybiotech Specifications Concentration 2X Detection Method SYBR Avoid multiple freeze-thawing of ROX solution The instrument settings for ROX reference dye are satisfactory for the measurement of the . Palliative Effect of Resveratrol against Nanosized Iron Oxide-Induced Oxidative Stress and Steroidogenesis-Related Genes Dysregulation in Testicular Tissue of Adult Male Rats. Maxima SYBR Green qPCR Master Mix: 10 min-95C 45 cycles of 30 sec-95C; 30 sec-55C; 30 sec-72C Melt Curve (60C to 95C) Total volume of 25 L containing 2 L of cDNA and 23 L of reaction mix (1x Maxima SYBR Green master mix, 0.4 M [1 M] each of pan-Lyssavirus primers) Genomic DNA up to 500 ng and plasmid DNA up to 10 ng can be used in qPCR with to light during handling to avoid lo ss of fluorescent signal intensity. usage. ZERO BIAS - scores, article reviews, protocol conditions and more The SYBR Green I intercalating dye allows for DNA detection and analysis without . Maxima is a SYBR is included in the master mix #K0241 #K0242 for 200 rxns . SYBR Green I is a fluorescent dye which binds all double-stranded DNA molecules and emits a fluorescent signal on binding. 8. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. 2720.00 6 SYBR SYBR Select Master Mix Cost-effective solution for real-time PCR applications. Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers Sensitivity - detects low copy number targets Wide linear range - accurate quantification across 9 orders of magnitude Bioz Stars score: 99/100, based on 1 PubMed citations. The LightCycler 480 SYBR Green I Master is a one-component hot start reaction mix for PCR. Use one positive sample and NTC per concentration. They are offered with a proprietary passive reference dye. Instead of 10um of both primers, you try PCR with 5um, 7.5um and 10um. The master mixes include Thermo Scientific Maxima Hot Start Taq DNA Polymerase in an optimized PCR buffer. The Maxima SYBR Green/ROX qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Shop Maxima Maxima English English; Change Country. Delivery cost : On demand . ZERO BIAS - scores, article reviews, protocol conditions and more liquid. Thermo Scientific Maxima SYBR Green qPCR Master Mixes are ready-to-use solutions optimized for qPCR. . Bioz Stars score: 99/100, based on 1 PubMed citations. SYBR SYBR Select Master Mix. PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye-based indicators for accurate reaction set up. Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. Sorry, no results matches for "SYBR Green qPCR Master Mix (No ROX)" Only template and primers need to be added. The Thermo Scientific Phusion High-Fidelity PCR Master Mixes are high performance enzymes now available in convenient master mix format. 7. Thermo Scientific Maxima SYBR Green/ROX qPCR Master Mix (2X) Ready-to-use solution optimized for qPCR and 2-step RT-qPCR. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers. Stock: Pre order. SYBR Green qPCR Supermixes. Only template and primers need to be added. NACRES: NA.55. Only template and primers need to be added. Supplied with nuclease-free water. RM. Thermo Scientific Maxima SYBR Green/ROX qPCR Master Mix (2X) Since the mix is provided as an easy-to-use real master reagent, reaction setup only requires the addition of template . Data are expressed as mean SD. Maxima SYBR Green/Fluorescein qPCR Master Mix (2x) is supplemented with fluorescein passive reference dye. Maxima SYBR Green qPCR Master Mix, no ROX. The master mix includes amfiSure Hot Start Taq DNA polymerase and dNTPs in an optimized PCR buffer. In International Journal of Environmental Research and Public Health on 4 July 2022 by Ahmed, M. M., Hussein, M. M. A., et al.. STORAGE . It's a 2X PreMix, taking advantage of a hot-start Taq polymerase combined with the antibody. Get All The Latest News Help & Support Center English English; Change Country; 0 . Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. It contains FastStart Taq DNA Polymerase and DNA double-strand-specific SYBR Green I dye for product detection and characterization. SG qPCR Master Mix (2x) contains dUTP, which partially replaces dTTP. 2x30 ml . Maxima Maxima SYBR Green/ROX qPCR Master Mix (2X) Ready-to-use solution optimized for qPCR and 2-step RT-qPCR. In addition, SYBR Green qPCR supermixes are available with either antibody-mediated hot-start Taq or Sso7d fusion polymerase, which provides increased stability for the polymerase-template complex, enhancing performance. Maxima Sybr Green Qrt Pcr Master Mix Kit, supplied by TaKaRa, used in various techniques. Shop Maxima Maxima English English; Change Country. 10. 4x12.5 ml 2x1.25 ml 10x1.25 ml . (K0222) Maxima SYBR Green/ROX qPCR Master Mix (2X) Sign in or register to save this reagent to your favourites Supplier Thermo Scientific Kit Type Nucleic acid amplification Assay Type Quantitative Detection Method Fluorometric View Product On Supplier's Website Request a Quote from Thermo Scientific Supplier provided information Validations Not for use in diagnostic procedures. MDL number: MFCD03458666. Maxima is a Maxima SYBR SYBR SYBR serve as an internal reference for normalization of the SYBR for 200 rxns . Related Products For Research Use Only. It contains SYBR Green I dye. Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers Sensitivity - detects low copy number targets Wide linear range - accurate quantification across 9 orders of magnitude Not for use in diagnostic procedures. 2 x 1.25 mL Maxima SYBR Green/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 L reactions. 845.49 Item details SKU: NEOSCI-K0251. The master mixes include Maxima Hot Start. Klotho mRNA expression was measured using the Maxima SYBR Green qPCR Master Mix. (K0223) Maxima SYBR Green/ROX qPCR Master Mix (2X) Supplier Thermo Scientific. I was using the Maxima SYBR Green/ROX qPCR Master Mix (2X) K0221 in the Rotor Gene 6000, and the Qiagen Master Mix doesnt have ROX. We offer you a combination of products, technical know-how . Gene expression was quantified by SYBR Green qPCR method using the Maxima SYBR Green/ ROX qPCR Master Mix on an StepOnePlus Real Time PCR system (ThermoFisher Scientific). Thermo Scientific Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) Ready-to-use solution optimized for qPCR and 2-step RT-qPCR. All Maxima SYBR Green qPCR Master Mixes are supplied with nuclease-free water. 13 Thermo Scientific ABsolute Blue QPCR Mix, SYBR Green, ROX For Maxima SYBR Green qPCR Master Mix, ROX Solution provide (2x), ROX is provided in a separate vial. Maxima SYBR Green qPCR Master Mix, ROX Solution provided (2x), comes with a separate ROX vial. Ceratocystis platani has been listed as a quarantine pest (EPPO A2 list) due to extensive damage caused in Southern Europe . RNA. Dessy-deilepaita- Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. Thermo Scientific Maxima SYBR Green/ROX qPCR Master Mix (2X), 1000 Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. SYBR Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. Quality Level. Ideal Taq polymerase and suitable buffer will guarantee preferable specificity . ZERO BIAS - scores, article reviews, protocol conditions and more DNA. Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. Nehemiah Center for Urban Leadership Development. 11. I have to agree with Thomas here. Kit Type Nucleic acid amplification Assay Type Quantitative Detection Method Fluorometric View Product On Supplier's Website Request a Quote from Thermo Scientific. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and SYBR Green dyeeverything needed for real-time PCR. Get All The Latest News Help & Support Center . The excitation and emission maxima of SYBR Green dye are at 494 nm and 521 nm, respectively, which are compatible with the use on any Real-Time cycler. Thermo Fisher maxima sybr green rox qpcr master mix Maxima Sybr Green Rox Qpcr Master Mix, supplied by Thermo Fisher, used in various techniques. Item Maxima SYBR Green/ROX qPCR Master Mix (2X) (1000 x 25 L rxns) Company Thermo Fisher Scientific; Catalog Number K0222; This product is no longer available on Biocompare. Browse a full range of Probe qPCR Master Mixes products from leading suppliers to meet your needs. Only template and primers need to be added. CONTENTS . In qPCR, DNA accumulates and fluorescent signal increases proportionally to the DNA concentration. Readjust the threshold value for analysis of every run. maxima tm sybr green rox qpcr master mix Roche ) 86 Roche maxima tm sybr green rox qpcr master mix Maxima Tm Sybr Green Rox Qpcr Master Mix, supplied by Roche, used in various techniques. Primer sequences for the quantification of 35 genes were purchased from Sigma and are . The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Get All The Latest News Help & Support Center English Deutsch; Change Country; 0 . Minimize exposure of Maxima SYBR Green qPCR Master Mix (2X), no ROX and ROX Solution . Thermo Scientific Oligo (dT)18 Primer. Find the best master mix by application. Maybe I should use the Maxima SYBR Green qPCR Master Mix (2X), ROX Solution provided in another tube K0251 OR K0252. The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. . Unlike reporter dyes in qPCR (e.g. Detection Method: SYBR: https://www.bioz.com/result/maxima sybr green fluorescein qpcr master mix/product/Thermo Fisher Average 97 stars, based on 1 article reviews Price from $9.99 to $1999.99 maxima sybr green fluorescein qpcr master mix - by Bioz Stars , 2022-09 97 / 100 stars Buy from Supplier NACRES: NA.55. The master mix contains: SYBr Green Dye . SYBR Green/ROX qPCR Master Mix (2X)-K0223-4000 reactTOYOBOAmrescoAnaspec Quality Level. Thermo Scientific Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial, 200 Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. 2 x 1.25 mL Nuclease-Free Water Store at -20C. Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof. Thermo Scientific Maxima SYBR Green/ROX qPCR Master Mix (2X), 200; Prev Next. Dodvate: Fermentas (Thermo Scientific) The Maxima SYBR Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Brilliant III Ultra-Fast SYBR Green QPCR Master Mix 1 Brilliant III Ultra-Fast SYBR Green QPCR Master Mix MATERIALS PROVIDED Catalog #600882 (single kit), #600883 (10-pack kit) Materials Provided Quantitya,b 2 Brilliant III Ultra-Fast SYBR Green QPCR Master Mixc, d 2 2 ml Reference dyed, 1 mM 100 l a Sufficient PCR reagents are provided for four hundred, 20-l reactions. dUTP is included in the mix for optional carry-over contamination control using UDG. Store at -20C in the dark for long term storage or at 4C for up to one month. The instrument settings for ROX reference dye are satisfactory for the measurement of the . Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers. liquid. of 25 l . All Maxima SYBR Green qPCR Master Mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer. 4 x 12.5 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 4000 x 25 L reactions. Bioz Stars score: 95/100, based on 1 PubMed citations. Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers. Shop now at Fisher Scientific. maxima sybr green fluorescein qpcr master mix ( Thermo Fisher ) 95 Thermo Fisher maxima sybr green fluorescein qpcr master mix Maxima Sybr Green Fluorescein Qpcr Master Mix, supplied by Thermo Fisher, used in various techniques. 2600.00 7 Maxima Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial Ready-to-use solution optimized for qPCR and 2-step RT-qPCR. Shop Maxima Maxima English Deutsch; Change Country. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Wide linear range - accurate quantification across 9 orders of . Many real time PCR protocols are moving away from SYBR Green because it inhibits PCR at higher concentrations . The master mixes include Maxima Hot Start. Thermo Scientific Maxima SYBR Green/ROX qPCR Master Mix (2X), 200 Out of stock. The master mix includes Maxima hot start Taq DNA polymerase, SYBR Green dye I and dNTPs in an optimised PCR buffer and is supplemented with ROX passive reference dye. 200. form. MDL number: MFCD03458666. for 1000 rxns for 4000 rxns of 25 l . Add to Wish List . Fastest qPCR kit in our portfolio with a speed of 40 min per 40 cycles Comes in a master mix format and includes a hotstart enzyme and ROX Low-abundance RNA, down to 10 fg Amplification and detection from total RNA, poly (A+), and synthetic RNA samples Reagents validated across a range of fast real-time PCR cyclers Return to top Specifications Properties. Relative expression was calculated by using the comparative Ct method (2-Ct). for 1000 rxns for 4000 rxns of 25 l . The master mix includes Maxima hot start Taq DNA polymerase, SYBR Green dye I and dNTPs in an optimised PCR buffer. Detection Method: SYBR: GC-Rich PCR Performance: The difference for such a problem is that the accumulation of a specific product can be directly seen from the amplification curve in the Taqman assay, whereas a SYBR Gree assay would require the . Our product 2X Green qPCR Master Mix provides superior specificity, robust amplification efficiency, ideal reproducibility and stability in quantifying target DNA or cDNA. The Maxima SYBR Green/ROX qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. 8. ROX, also known as carboxyrhodamine, is an inert fluorescent dye that can be added to the qPCR master mix. Properties. SYBR Green), ROX is referred to as a passive dye, since its fluorescence signal is not influenced by the PCR reactions. Hot start PCR master mix, sybr green PCR master mix, sybr green qPCR. Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers Sensitivity - detects low copy number targets Wide linear range - accurate quantification across 9 orders of magnitude Thermo Scientific Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial. Brand origin : United States . Thermo Scientific . Introduction amfiSure SYBR Green qPCR Master Mix (5X) is a ready-to-use solution optimized for quantitative real-time PCR and two-step real-time RT-PCR. Delivery time : 1 Week/s . DESCRIPTION. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers. P Techniques Used: Expressing, In Vitro, SYBR Green Assay, Real-time Polymerase Chain Reaction . Rating: 0 out of 100 - based on 0 review(s) | Write Review | Add to Compare . Supplied with nuclease-free water. Maxima Maxima SYBR Green/ROX qPCR Master Mix (2X) Ready-to-use solution optimized for qPCR and 2-step RT-qPCR. View PDF. Order number : K0221 of 25 l Maxima SYBR Green/ROX qPCR Master Mix (2X) 2x1.25 ml 10x1.25 ml . SYBR Green qPCR Master Mix (2X), ROX solution provided-K0253-4000 reactNorgen biotekMilliporeCST Data are normalized to GAPDH mRNA expression levels ( n = 3 per group). SYBR Green qPCR supermixes are compatible with most protocols for qPCR. ZERO BIAS - scores, article reviews, protocol conditions and more of 25 l Maxima SYBR Green/Fluorescein qPCR . 200. form. Bioz Stars score: 86/100, based on 1 PubMed citations. Specifications Concentration Detection Method Format STORAGE . Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. page SYBR Green/Fluorescein . The master mix includes Maxima hot start Taq DNA polymerase, SYBR Green dye I and dNTPs in an optimised PCR buffer and is supplemented with ROX passive reference dye. The master mixes include Maxima Hot Start. Store at -20C in the dark. Brand: Thermo Scientific. Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. Thermo Scientific Maxima Probe/ROX qPCR Master Mix (2X) Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes. Add to Procurement List . Maxima SYBR Green/ROX qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Thermo Fisher maxima sybr green rox qpcr master mix Maxima Sybr Green Rox Qpcr Master Mix, supplied by Thermo Fisher, used in various techniques. In addition, excellent results have been observed using most other QPCR platforms. Only template and primers need to be added. Expiry time : On demand . Related Products For Research Use Only. 12 Thermo Scientific DyNAmo HS SYBR Green qPCR Kit Contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR. The master mixes include Maxima Hot Start. Maxima SYBR Green/ROX qPCR Master Mix (2X) usage. 2x1.25 ml 10x1.25 ml . Shop Maxima Maxima The Brilliant II SYBR Green QPCR master mix has been optimized for maximum performance on the Agilent Mx3000P and Mx3005P real-time PCR systems and Agilent Mx4000 multiplex quantitative PCR system, as well as on the ABI 7900HT real-time PCR instrument. Maxima SYBR Green/ROX qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Applied Biosystems SYBR Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. Unit size : 200 Reactions . Bioz Stars score: 97/100, based on 1 PubMed citations. The excitation and emission maxima of SYBR Green I are at 494 nm and 521 nm, respectively, which are compatible with use on any real-time cycler. Ceratocystis platani (CP), an ascomycetous fungus, is the agent of canker stain, a lethal vascular disease of Platanus species. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers. ZERO BIAS - scores, article reviews, protocol conditions and more Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 1,000 x 25L rxns: TH K0253: Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 4,000 x 25L rxns: Westburg is an independent distributor providing high quality, innovative products to Life Sciences laboratories. The master mixes include Maxima Hot Start Taq . The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and of course, SYBR Green dyeeverything needed for real-time PCR, except the sample and PCR primer pair. 7. Sensitivity - detects low copy number targets. Store at -20C in the dark for long term storage or at . 4x12.5 ml Master Mix (2X) 2x1.25 ml 10x1.25 ml 2x30 ml . Thermo Scientific ABsolute Blue QPCR Mix, SYBR Green, low ROX. Maxima Maxima SYBR Green/ROX qPCR Master Mix (2X) Ready-to-use solution optimized for qPCR and 2-step RT-qPCR. Hot start PCR master mix, sybr green PCR master mix, sybr green qPCR.
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